Description
The CA-FLUOM-5B benchtop fluorometer is easy to operate, combined with a highly sensitive quantitative analysis kit, to accurately quantify DNA, RNA and protein concentrations. And it is equipped with two channels and has the ability to quickly analyze two fluorescent signals of one sample, which is economical!
The combination of the SP-MUV1000 Micro-Volume Spectrophotometer and the CA-FLUOM-5B Fluorometer provides comprehensive, accurate, easy and fast quantification of biomolecules.
Product features
- Simple and intuitive – 7.0 inch color touch screen.
- Fast detection – fast and accurate quantification of DNA, RNA and protein in 3 seconds.
- High sensitivity – the lowest detection limit can reach 0.5pg/μL double-stranded DNA.
- Configure two fluorescence channels: Measure two different fluorescence in one analysis.
- Five orders of magnitude response range.
- Open system, which can match common reagents on the market.
- More than 100,000 sample results can be stored, and can be exported through U disk.
- Micro adapter: 0.5ml quantitative PCR centrifuge tube adapter (standard configuration); 0.2ml quantitative PCR centrifuge tube adapter (optional).
Fluorometers are especially suitable for:
- Your samples are rare and difficult to process
- Based on fluorescence detection technology, it is three orders of magnitude higher than traditional UV spectrophotometry.
- The sample will be used for expensive downstream experiments: qPCR, PCR cloning, transfection and next-generation sequencing and other precision assays
- Ultra-low concentration samples (10pg/μL dsDNA).
PRODUCT DETAILS
SPECIFICATIONS
Performance index
light source | LED |
Dynamic range | 5 orders of magnitude |
Repeatability | <1.5% |
stability | <1.5% |
Linearity | R2>0.995 |
Sensitivity | dsDNA:0.5ng/ml |
Measuring speed | <3s(Master single test) |
Packaging Size | 440*270*160mm |
Net/ Gross Weight | 2/ 2.5 kg |
Standard configuration: channel
Channel | Excitation wavelength | Emission wavelength |
Blue | 470nm | 525nm |
RED | 625nm | 690nm |
Optional channel
Channel | Excitation wavelength | Emission wavelength |
UV | 365nm | 460nm |
GREEN | 525nm | 620nm |
Commonly used fluorescent reagents corresponding to different fluorescent channels and their applications
Excitation wavelength | Common fluorescent reagents | Application |
365 | Hoechst33258, 4-MU,EnZCheK Caspase | Plant GUS reporter gene assay, cell apoptosis assay |
470 | PicoGreen®, oligreen,RiboGreen®, GFP, Protein, Quant-iT™ | dsDNA, ssDNA quantification, green fluorescent protein GFP, protein quantification |
525 | Rhodamine, Cy3, RFP Vybrant Cytotoxicity | Rhodamine detection, Cy-3 fluorescent label detection, red fluorescent protein RFP gene detection, cell activity toxicity detection |
625 | Cy5, Quant-iT RNA | Cy-5 fluorescent labeling detection, RNA quantification |
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